Segmenting an example FLIM image with the phasor plot in napari#

  • Open the hazelnut 2D, hazelnut 3D or the synthetic lifetime cat sample data from the napari-flim-phasor-plotter under File > Open Sample > napari-flim-phasor-plotter

  • Calculate the phasor plot under Plugins > napari-flim-phasor-plotter > Calculate Phasors

  • Manually select clusters with the mouse and check the segmentation results on the main canvas.

  • Re-calculate the phasor plot exploring other parameters (median, harmonic, threshold) to improve clusters display in the phasor plot.